BC10: Microscopy (6 ECTS)

STADS: 3040601

Level


Teaching period

Autumn semester.

Teacher responsible
No responsible teachers found, contact the department if necessary

Timetable
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Prerequisites:
None

Academic preconditions:
Cell Biology and Histology (BC 01).

Course introduction
The object is to provide a general view of the instruments and methods one can resort to when faced with a whole microorganism, an organelle in a cell, a metal surface of just anything small, which you would like to see enlarged and maybe study the composition of.

Expected learning outcome


Subject overview
The principles behind the different types of light-microscopes are described and practical exercises carried out with as many methods as possible. Since it can become necessary to pretreat the objects to be examined, methods for preparation of samples will also be described.
Today, electronmicroscopes have become a common tool for examining objects at higher magnifications than the lightmicroscopes can provide. As a consequence of this, the function of the two main types of electronmicroscopes will be described, and the participants will get the opportunity to use both types of microscopes. The particular methods for preparing samples for electronmicroscopes will be described, as will be the risks of creating artefacts (false structures created during the preparation). Both light- and electronmicroscopes can be used for measuring physical dimensions of small objects, as it will be tried out during the practical exercises. The chemical composition of an object can be investigated by x-ray microanalysis, which will be demonstrated. Since the technical development points at a replacement of the classical photographs by digitized images, the course will offer opportunities to work with enlarged, digitized images, which can be manipulated in a computer.

Literature

Mikroskoper og metoder - en oversigt. OU 1998.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Approval of report. Pass/fail basis. Internal examiner.

Expected working hours
The teaching method is based on three phase model.

Forelæsninger, demonstrationer og praktiske øvelser på små hold.
Educational activities

Language
No recorded information about the language used in the course.

Remarks
Please remember to register for examination/evaluation. The course is held by Institute of Medical Biology Anatomy and Neurobiology.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
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