BM117: Modern Techniques in Cell Culturing and Signal Transduction (6 ECTS)

STADS: 102041

Level


Teaching period

Spring semester.

Teacher responsible
No responsible teachers found, contact the department if necessary

Timetable
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Comment:
Laboratorieøvelser i uge 17 (onsdag-fredag) og uge 18

Prerequisites:
None

Academic preconditions:
The participants are expected to have passed courses in elementary biochemistry and molecular biology. Biochemistry A (BK05), Biochemistry B (BK06), Protein- and Enzyme Chemistry (BX05) and Molecular Biology A (BM05).

Course introduction
To give an introduction to basic cell culture techniques and modern methods used to investigate cell functions and signal transduction pathways. Eukaryotic cell lines will serve as model systems to understand how inactivation of genes can be used to unravel the interplay and interaction between various components of signaling cascades. In addition, participants will gain experence with a range of biochemical and molecular biological techniques.

Expected learning outcome


Subject overview
Culturing of a eukaryotic cell line, commonly used sterile techniques, PCR techniques, transfection of cell cultures, experimental approaches, i.e. SDS-PAGE and Western blotting, to understand the function of different components in signaling cascades

Literature

Udvalgte artikler.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Written report, external examiner. Marks according to the Danish 13-point marking scale.

Expected working hours
The teaching method is based on three phase model.

Forelæsninger (6 timer) og laboratorieøvelser (54 timer)
Educational activities

Language
No recorded information about the language used in the course.

Remarks
Max. 8 participants

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.