BM25: Advanced Experimental Molecular Biology (12 ECTS)

STADS: 1653101

Level


Teaching period

Spring semester. ( August)

Teacher responsible
Email: s.mandrup@bmb.sdu.dk

Timetable
There is no timetable available for the chosen semester.

Prerequisites:
None

Academic preconditions:
BM111 Eukaryot Molecular Biology (BM111) must be passed or followed simultaneously.

Course introduction
The objectives of the course are:
1) to give a theoretical introduction to the molecular processes leading to differentiation of eukaryotic cells (differentiation of adipocytes in vitro is used as a model system);
2) to give a practical introduction to several of the most commonly used techniques in molecular biological analyses of gene expression in higher eukaryotic cells. Furthermore, the course aims at giving the participants insight into how bachlor/ITEK/"speciale"-related research projects are planned and conducted. The course is therefore a combination of lectures, presentations of relevant papers and intensive laboratory work where the students are actively involved in the planning of experiments.

Expected learning outcome


Subject overview
Theoretical introduction to adipocyte differentiation with special emphasis on transcriptional regulation.
Analysis of gene expression during in vitro differentiation of 3T3-L1 cells to adipocytes. This includes investigations of the expression of important transcription factors and adipocyte-specific genes.

Techniques:
Purification of RNA.
Northern blotting and hybridization.
Isolation of DNA fragments.
Multiplex RT+PCR
RNase protection.
Western blotting.
Electrophoretic mobility shift assay.

Literature

Ny øvelsesvejledning hvert år.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Approval of report. Internal examiner. Pass/fail.

Expected working hours
The teaching method is based on three phase model.

Laboratory course (90 hours) and lectures and presentation of papers (30 hours). Intensive course, where long days (10-12 hours) can be expected some of the days.
Educational activities

Language
No recorded information about the language used in the course.

Remarks
The laboratory manual is updated every year. Remember registration as a spring semester course. The course is only taught in Danish.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.