BMB207: Biophotonics (5 ECTS)

STADS: 01015201

Level
PhD course

Teaching period
The course is offered in the spring semester.

Teacher responsible
Email: brewer@memphys.sdu.dk

Timetable
There is no timetable available for the chosen semester.

Comment:
Max deltagerantal: 16. Kurset kører i januar. Kurset er et ph.d.-kursus. Kandidatstuderende kan tage kurset iflg. forudgående aftale med underviser. Afholdes i januar 2018.

Prerequisites:
Master degree in biology, chemistry, physics, bio chemistry and molecular biology, biomedicine, medicine or nanobioscience.

Academic preconditions:
None

Course introduction
It is the goal of the course to teach the students general principles of fluorescence and light microscopy as well as teach principles behind advanced fluorescence and imaging techniques as well as a short introduction to image processing. There will be practical demonstrations and hands on exercises on all topics. The course is a five day intensive course taking place within one week.

In relation to the competence profile of the degree it is the explicit focus of the course to:

  • Give the competence to Identify the “method of choice” for analysis of a given biological sample
  • Give skills to perform image analysis using advanced microscopy instrumentation
  • Give knowledge and understanding of the strengths and limitations of modern techniques in microscopy

 



Expected learning outcome
The learning objective of the course is that the student demonstrates the ability to:
  • Understand the basics of microscopy and optical bioimaging.
  • Discuss strengths and limitations of modern techniques in microscopy.
  • Identify the “method of choice” for analysis of a given biological sample
  • Perform image analysis using advanced microscopy instrumentation 
  • Present scientific results in an oral seminar given in English
  • Evaluate scientific results in the original research literature
 


Subject overview
The following main topics are contained in the course:
  • Wide field microscopy
  • Laser scanning single-photon confocal microscopy
  • Two-photon microscopy
  • Spinning disk confocal microscopy
  • Fluorescence Correlation Spectroscopy (FCS)
  • Raster Image Correlation Spectroscopy (RICS)
  • Fluorescent Lifetime Imaging microscopy (FLIM)
  • kICS/PALM (k‐Space Image Correlation Spectroscopy/ photoactivated localization microscopy) 
  • Coherent anti Raman scattering microscopy (CARS) 
  • Stimulation emission depletion microscopy (STED)
  • Introduction to image analyses using FIJI and Huygens. 
  •  
 


Literature
There isn't any litterature for the course at the moment.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
  1. Deltagelse i alle forelæsninger, Laboratorieøvelse og eksaminatorier timer. Bestået/ikke-bestået, intern censur ved underviser. (01015212).
Assessment and marking:
  1. Mundtlig eksamen, intern bedømmelse ved underviser, bestået/ikke bestået. Nærmere beskrivelse af eksamensreglerne vil blive offentliggjort under 'Course Information' på kursets side i Blackboard.(5 ECTS). (01015202).
Expected working hours
The teaching method is based on three phase model.
Intro phase: 12 hours
Skills training phase: 29 hours, hereof:
 - Tutorials: 8 hours
 - Laboratory exercises: 21 hours

Educational activities
  • Literature studies
  • Preparation of project individually and in groups
 
Educational form

Language
This course is taught in English.

Remarks
Kurset kan ikke tages hvis man allerede har taget BMB825. 

The course has limited entry with a maximum of 18 students. The following 4 criterias are taken into consideration when seats are assigned.
  1. Students with the most ECTS from their master
  2. Students who are accepted conditionally on the master 
  3. Students who follows master courses concurrent with their bachelor programme
  4. Bachelor students
If the score is even lots are drawn.

The academic enviroments at the Faculty of Science manages the prioritisation and at waiting list is established and will then be made aware from the faculty. The waiting list will not be transferred to the following year.

In order to keep the assigned seat you need to attend the first course day or notify the teacher, otherwise the seat is given to the next student from the waiting list.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.