English

BMB825: Bioimaging (5 ECTS)

STADS: 01011801

Level
Master's level course

Teaching period
The course is offered in the spring semester.

Teacher responsible

Email: brewer@memphys.sdu.dk

Additional teachers

wuestner@bmb.sdu.dk

Timetable

Group	Type	Day	Time	Classroom	Weeks	Comment
Common	I	Monday	12-14	U142	17
Common	I	Monday	12-14	U70	18
Common	I	Monday	08-10	U70	19
Common	I	Tuesday	12-14	U10	16
Common	I	Tuesday	10-12	U70	20
Common	I	Wednesday	08-10	U70	18
Common	I	Wednesday	12-14	U70	19
Common	I	Thursday	12-14	U142	16
Common	I	Thursday	12-14	U70	17
H1	TL	Monday	12-16	*Odense Lokalitet aftales 11	19	Spørg underviser om lokale
H1	TE	Monday	10-12	U70	22
H1	TE	Tuesday	12-14	U70	20-21
H1	TL	Tuesday	12-16	*Odense Lokalitet aftales 11	22	Spørg underviser om lokale
H1	TE	Wednesday	12-14	U70	17
H1	TL	Wednesday	08-12	*Odense Lokalitet aftales 11	20	Spørg underviser om lokale
H1	TL	Wednesday	09-13	*Odense Lokalitet aftales 11	21	Spørg underviser om lokale
H1	TL	Friday	08-12	*Odense Lokalitet aftales 11	18	Spørg underviser om lokale

Show entire timetable
Show personal time table for this course.

Comment:
MAX 12 studerende

Prerequisites:
None

Academic preconditions:
Bachelors degree in either Biochemistry and molecular biology, Nanobioscience or Physics and Chemistry. Due to instument availability a maximum of 12 participants can enroll in the course. For BMB students it is advised to have BMB509 passed

Course introduction
In the last years, a large number of state of the art experimental microscopy techniques have been developed. The main objective of this course is to provide the theoretical basis of these techniques together with some “hands on” laboratory practice.

Expected learning outcome
At the end of the course the student is expected to :

Evaluate scientific results in the original research literature
Present scientific results in an oral seminar given in English
Discuss strengths and limitations of modern techniques in microscopy
Identify the “method of choice” for analysis of a given biological sample
Perform image analysis using advanced microscopy instrumentation

Subject overview
Basic theory (including quantitaive image analysis) for the following techniques will be provided: 1) Laser Scanning Confocal Fluorescence microscopy 2) Spinning Disk Confocal Fluorescence microscopy 3) Widefield Fluorescence microscopy 4) Multiphoton Excitation Fluorescence microscopy 5) Second Harmonic Generation microscopy 6) Fluorescence Lifetime imaging and FRET 7) Super resolution techniques (STED microscopy) 8) Fluorescence Correlation spectroscopy related techniques (FCS, RICS)

Literature

Oplyses senere.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Oral exam, Danish 7 mark scale, internal examiner.
Lab.work, pass/fail,internal evaluation by teacher. (01011802)

Remaks regarding evaluation:

For the lab exercise, the students will be separated in 4 groups of 3 persons and work in teams in four different exercises (1 per group). Each group need to report and discuss their results to the rest of the class in a seminar. This activity will be evaluated, pass/fail grading by the teacher (1 ECTS); (01011812)
Oral examination. Grades by the 7 mark scale, internal examinator (4 ECTS). (01011802)

Reevaluation at the latest 6 weeks after the ordinary exam.

Expected working hours
The teaching method is based on three phase model.

Forelæsninger, antal timer 24
Eksaminatorier/opgaveregning, antal timer 4
Laboratorieøvelser, antal timer 20
Educational activities

Language
This course is taught in English.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.