BB504: Eksperimental methods (5 ECTS)
STADS: 04000401Level
Bachelor course
Teaching period
The course is offered in the autumn semester.
1st quarter.
Teacher responsible
Email: klp@biology.sdu.dkTimetable
| Group | Type | Day | Time | Classroom | Weeks | Comment |
|---|---|---|---|---|---|---|
| Common | I | Monday | 08-09 | U144 | 37-41 | |
| S1 | TE | Monday | 09-10 | U144 | 37-41 | |
| S1 | TL | Monday | 10-14 | Biologi | 37-41 | |
| S1 | TL | Wednesday | 14-18 | Biologi | 37-41 | |
| S3 | TL | Wednesday | 08-12 | Biologi | 37-41 | |
| S3 | TL | Friday | 14-18 | Biologi | 37-41 |
Show personal time table for this course.
Prerequisites:
None
Academic preconditions:
None.
Course introduction
The purpose of the course is to enable the student to perform laboratory work, including sterile-lab work with cell- and bacterial cultures, in a safe and independent way.
Expected learning outcome
After the course the student is expected to be able to
• Perform quality control on basic laboratory equipment and calibrate pH electrodes and microscopes
• Make solutions of chemicals from the ‘Materials and Methods’ section of articles
• Plan and perform quantitative measurements using standard curves and standard addition methods
• Extract chemicals from water samples using affinity chromatography
• Report results using basal statistical methods
• Work according to protocols for sterile laboratories
• Work according to safety regulations in the lab including being able to classify and dispose chemical waste
• Handle liquid nitrogen and pressure flasks properly
Subject overview
GLP, quality assessment and certainty of measurements.
Calibration, linearity, extinction coefficients, calculation of concentrations and molarities.
Use of electrodes and spectroscopy
Quantification, standard curves and standard addition
Sterile lab work
Laboratory safety
Chemical data sheets, labelling of chemicals, KIROS
Disposal of chemical and biological waste
Literature
There isn't any litterature for the course at the moment.
Website
This course uses e-learn (blackboard).
Prerequisites for participating in the exam
None
Assessment and marking:
Approval of report (pass / fail). Internal marking by teacher.
Re-examination after 2nd quarter
Expected working hours
The teaching method is based on three phase model.
Forelæsninger (5 timer), eksaminatorier (5 timer), laboratorieøvelser (40 timer).
Educational activities
Language
This course is taught in Danish.
Course enrollment
See deadline of enrolment.
Tuition fees for single courses
See fees for single courses.
