BB93: Knowledge in Laboratory Animal Science B (4 ECTS)

STADS: 407141

Level


Teaching period

Autumn semester.

Teacher responsible
No responsible teachers found, contact the department if necessary

Timetable
There is no timetable available for the chosen semester.

Prerequisites:
None

Academic preconditions:
Biology students at SDU cannot follow this course, but must follow BB83 instead.

Course introduction
The aim of the course is to give students sufficient knowledge about work with laboratory animals to allow them (under the supervision of an animal experiment licence holder) to participate in experiments that are covered by the law regarding animal experiments.

Expected learning outcome


Subject overview
Lectures:
Legislation and ethical aspects of animals experiments (1½ lecture).
Animal models, experimental design and alternative models (1½ lecture): Animal models in general. The “three Rs”. Guidelines regarding housing and animal care. Planning of experiments (experimental design and statistics). Use of cell cultures and other alternatives.
Animal welfare (1 lecture): Normal behaviour. Stress behaviour. Laboratory animal facilities. Environmental enrichment. Health monitoring.
Nutrition (1 lecture): Nutritional requirements. Types of diets. Nutritional allowance, feeding intervals etc.
Anesthesia, analgesia and euthanasia (2 lectures): Types of anaesthetics in different classes of vertebrates. Practical aspects of anaesthesia. Degrees of anaesthesia. Surgery. Postoperative treatment. Analgesia. Methods for euthanasia in different vertebrates.
Mice, rats and other mammals as animal models (2 lectures): Handling of mice and rats. Genetics. Health monitoring. Sampling. Surgery. Analgesia, anaesthesia etc.
Fish as experimental animals (2 lectures): Fish as models in biological research. The “August Krogh Principle” and specific examples of fish models. Transport and housing. Aquaria types. Water quality and critical water parameters. Disease prevention and treatment. Pain and stress in fish. Handling. Anaesthesia. Sampling, injections and surgical techniques. Euthanasia.
Amphibia and reptiles as experimental animals (1 lecture): Use of amphibia and reptiles in biological research. Housing and nutrition. Handling, sampling, injections and surgical techniques. Euthanasia.
Toxicological and ecotoxicological experiments (1 lecture): Considerations in (eco)toxicological experiments with fish and other animals.

Practicals:
Fish: Handling, anaesthesia, sampling of blood, IP-injection etc. (2 h)
Frogs: Handling, anaesthesia, dissection, making a muscle-nerve preparation (2 h).
Mammals: practical handling of rodents. Anaesthesia. Sampling etc. Dissection of elasmobranch (3 h)
Dissection of teleost (3 h)
Dissection of bird (3 h)
Dissection of mouse (3 h)
Comparative anatomy of skeleton, respiration and circulation (3 h)

Literature
There isn't any litterature for the course at the moment.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Active participation in the course. 2 h written exam evaluated on a pass/fail basis. Internal examiners. All course material allowed at the exam.

Expected working hours
The teaching method is based on three phase model.

13 h lectures.
22 h laboratory practicals.
Educational activities

Language
No recorded information about the language used in the course.

Remarks
The course originates in proclamation 1016 of 12. December 2001 that concerns the demands for qualifications of persons working with experimental animals.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.