BMB202: Protein Purification (10 ECTS)

STADS: 01003901

Level
PhD course

Teaching period
The course is offered in the autumn semester.
Autumn semester.

Teacher responsible
Email: nils.f@bmb.sdu.dk

Timetable
Show entire timetable
Show personal time table for this course.

Comment:
Max 12 og min. 8 på hvert hold i lab. pr. uge (ca. 60 øvelsestimer pr. uge).
Øvelserne i uge 43. (Lokale: BMBs øvelseslokale).
Holdinddeling til laboratorieøvelserne finder sted ved forelæsningerne

Prerequisites:
None

Academic preconditions:
Passed bachelors degree. The course may be taken by master's students however, please notice that this is a PhD course and thus the level is very high.

Course introduction
To introduce participants to the theory and use of classical protein purification methods.

Expected learning outcome


Subject overview
The course consists of a theoretical part where the theory behind the methods used is discussed, and a practical part. In the practical part the students will purify a protein. The methods used include cell organelle separation by centrifugation, salt precipitation, ion exchange chromatography, gel filtration, protein determination and photometric enzyme assay. The participants are expected to document purity and to determine molecular weights using gel electrophoresis.

Literature

• Robert K, Scopes: Protein Purification - Principles and Practice, Springer Verlag, 3. udgave.
• Udvalgte forsknings- og oversigtsartikler.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Approval of report followed by an oral examination. External examiner. Grades according to the Danish 7-point scale. The report may be worked out in groups of two students. The examination may also be in groups of two - with individual evaluation.

Expected working hours
The teaching method is based on three phase model.

Forelæsninger og laboratorieøvelser.
Educational activities

Language
This course is taught in English, if international students participate. Otherwise the course is taught in Danish.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.