BMB202: Protein Purification (10 ECTS)

STADS: 01012201

Level
PhD course

Teaching period
The course is offered in the autumn semester.

Teacher responsible
Email: nils.f@bmb.sdu.dk

Timetable
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Comment:
Max 12 og min. 8 på hvert hold i lab. pr. uge (ca. 60 øvelsestimer pr. uge). Holdinddeling til laboratorieøvelserne finder sted ved forelæsningerne. Øvelserne afholdes i hele uge 43.

Prerequisites:
Passed bachelor's degree.

Academic preconditions:
The course may be taken by master's students however, please notice that this is a PhD course and thus the level is very high.

Course introduction
To introduce participants to the theory and use of classical protein purification methods.

Expected learning outcome


Subject overview
The course consists of a theoretical part where the theory behind the methods used is discussed, and a practical part. In the practical part the students will purify a protein. The methods used include cell organelle separation by centrifugation, salt precipitation, ion exchange chromatography, gel filtration, protein determination and photometric enzyme assay. The participants are expected to document purity and to determine molecular weights using gel electrophoresis.

Literature

• Robert K, Scopes: Protein Purification - Principles and Practice, Springer Verlag, 3. udgave.
• Udvalgte forsknings- og oversigtsartikler.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
Participation in the experimental part and hand in of a lab report is a prerequisite for attending the oral exam. The report must be handed in 3 weeks after the last day of exercises. Pass/fail, internal evaluation by teacher.

Assessment and marking:
Oral examination based on the lab report. External examiner. Grades according to the Danish 7-point scale. The examination may also be in groups of two - with individual evaluation.

Reexam in the same exam period or immediately thereafter.

Expected working hours
The teaching method is based on three phase model.
Intro phase: 10 hours
Skills training phase: 60 hours, hereof:
 - Laboratory exercises: 60 hours

Educational activities Study phase: 90-100 hours

Language
This course is taught in English, if international students participate. Otherwise the course is taught in Danish.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.