BMB509: Bioanalytical instrumentation (5 ECTS)

STADS: 01000901

Level
Bachelor course

Teaching period
The course is offered in the autumn semester.
Second quarter.

Teacher responsible
Email: wuestner@bmb.sdu.dk

Additional teachers
bagatolli@memphys.sdu.dk
tjdj@bmb.sdu.dk

Timetable
Group Type Day Time Classroom Weeks Comment
Common I Monday 10-12 U148 46-51
Common I Tuesday 08-10 U148 46-51
Common I Friday 08-10 U148 46-48
S1 TL Wednesday 12-16 48-51
S1 TL Thursday 14-18 47
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Revison of timetable:
: Lab uge 47 flyttet til torsdag kl. 14-18.

Prerequisites:
None

Academic preconditions:
BMB506 Protein chemistry, BMB502 Enzymes and membranes, BMB504 Fundamental Molecular Biology and BMB503 Fundamental Biochemistry must be passed.

Course introduction
Give students an understanding of selected biochemical techniques to determine biomolecular structure and function as well as spatial distribution of biomolecules and molecular complexes in cells.
Give the principles of - and practical experience with - separation and characterization techniques for isolation of macromolecules.
Introduce absorption and fluorescence spectroscopy, fluorescence microscopy and mass spectrometry methods used in modern biochemistry and molecular cell biology, including practical work and demonstrations.

Qualifications
.

Expected learning outcome


Subject overview
• Introduction to electromagnetism and optics of relevance to bioanalytical instrumentation used by biochemists and molecular cell biologist.
• Separation of biomolecules by physicochemical properties by chromatography or electrophoresis
• Introduction into light absorption and fluorescence phenomena as basis for fluorescence spectroscopy and microscopy
• Detection and characterization of biomolecules by microscopy, spectroscopy, mass spectrometry
• Visualization and localization of biomolecules in cells by microscopy techniques.

Literature
  • Keith Wilson and John Walker: Principles and Techniques of Biochemistry.


Syllabus
See syllabus.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Report: Research proposal that describes a protocol for the isolation and characterization of biomolecule of own choice. Marks according to the Danish 7-point scale and internal examiner.

Expected working hours
The teaching method is based on three phase model.

30 forelæsninger. 20 laboratorieøvelser.
Educational activities

Language
This course is taught in English.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.