BMB509: Bioanalytical instrumentation (5 ECTS)

STADS: 01012501

Level
Bachelor course

Teaching period
The course is offered in the autumn semester.

Teacher responsible
Email: wuestner@bmb.sdu.dk

Additional teachers
bagatolli@bmb.sdu.dk
tjdj@bmb.sdu.dk

Timetable
Group Type Day Time Classroom Weeks Comment
Common I Monday 10-12 U140 46-48,50
Common I Monday 10-12 U20 49
Common I Tuesday 10-12 U140 45-46
Common I Wednesday 12-14 U20 45
Common I Thursday 10-12 U140 45-46,48
Common I Thursday 08-10 U140 47,49
H1 TE Monday 08-10 U133 46-47
H1 TL Tuesday 12-16 White Lab 47-50
H2 TE Tuesday 08-10 U43 46-47
H2 TL Friday 12-16 White Lab 47-50
H3 TL Monday 12-16 White Lab 47-50
H3 TE Wednesday 08-10 U17 46-47
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Comment:
Max 20 pr. øvelseshold

Prerequisites:
None

Academic preconditions:
BMB532 Fundamental Biochemistry and BMB533 Molecular biology and protein chemistry assumed known. Alternatively BMB506 Protein chemistry, BMB502 Enzymes and membranes, BMB504 Fundamental Molecular Biology and BMB503 Fundamental Biochemistry assumed known.

Course introduction
Give students an understanding of selected biochemical techniques to determine biomolecular structure and function as well as spatial distribution of biomolecules and molecular complexes in cells.
Give the principles of - and practical experience with - separation and characterization techniques for isolation of macromolecules.
Introduce absorption and fluorescence spectroscopy, fluorescence microscopy and mass spectrometry methods used in modern biochemistry and molecular cell biology, including practical work and demonstrations.

Qualifications
.

Expected learning outcome


Subject overview
  • Introduction to microscopical optics and computer-assisted image analysis
  • Separation of biomolecules based on physicochemical properties by chromatography or electrophoresis
  • Introduction into light absorption and fluorescence phenomena as basis for fluorescence spectroscopy and microscopy
  • Detection and characterization of biomolecules by microscopy, spectroscopy, mass spectrometry
  • Visualization and localization of biomolecules in cells by microscopy techniques.
Literature
    Noter og artikler


Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
Lab erxercises, pass/fail, internal evaluation by the teacher.

Assessment and marking:
  1. Oral exam with marks according to the Danish 7-point scale and internal examiner.

Re-examination in same term



Expected working hours
The teaching method is based on three phase model.

30 h forelæsninger, 4 eksaminatorietimer og 16 h laboratorieøvelser.
Educational activities

Language
This course is taught in Danish or English, depending on the lecturer.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.