BMB515: Biomolecular mass spectrometry (5 ECTS)

STADS: 01001501

Level
Bachelor course

Teaching period
The course is offered in the spring semester.
4th quarter.

Teacher responsible
Email: jenseno@bmb.sdu.dk

Additional teachers
f.kir@bmb.sdu.dk

Timetable
Show entire timetable
Show personal time table for this course.

Comment:
Maksimalt deltagerantal 24.

Prerequisites:
None

Academic preconditions:
Passed Science year for SDU students. Knowledge of basic and organic chemistry, biochemistry, protein chemistry and molecular biology is assumed. The course cannot be follow by students who have passed BMK21, BM31 or BM32 on the previous study programme.

Course introduction
Introduction to the use of mass spectrometry in chemistry, biochemistry, biomedicine, molecular biology and systems biology (genomics/proteomics/metabolomics). The emphasis will be on analysis of proteins and nucleic acids, but with aspects of lipids, carbohydrates and metabolites.

Qualifications
By the end of the course, the student can:
Give the outline of modern mass spectrometers, including tandem mass spectrometers
Interpret simple mass spectra and tandem mass spectra
Give motivated suggestions for mass spectrometric analysis of simple chemical or biochemical/molecular biology problems, including:
• Motivation for the choice of mass spectrometric instrumentation for a given molecule
• Motivation for the choice of sample preparation before mass spectrometric analysis.
Read, understand and evaluate the use of mass spectrometry in chemical and biochemical/molecular biology research literature.

Expected learning outcome


Subject overview
Introduction to modern mass spectrometric techniques, including different types of ionisations and mass analysers.
Instrument aspects and fragmentation techniques.
Structure elucidation of biological molecules by mass spectrometry and tandem mass spectrometry.
Combinations of chromatography and electrophoresis with mass spectrometry.
Quantitative analysis of biomolecules by mass spectrometry.
Metabolic studies using stable isotopes.
Sample preparation for mass spectrometry.

Literature

• E. De Hoffmann, V. Stroobant : : Mass Spectrometry - principles and applications, , (3nd Ed., Wiley 2007 ). (kan købes i Studenterboghandelen). .

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Written exam with marks according to the Danish 7-scale.
All written aids except computers may be used during exam.
Active participation in the lab exercises is required to pass the course.

Expected working hours
The teaching method is based on three phase model.

Forelæsninger (34 timer) og laboratorieøvelser (16 timer). Forelæsningerne inkluderer opfølgning på laboratorieøvelser, opgaveregning og artikelgennemgang.
Educational activities

Language
This course is taught in English, if international students participate. Otherwise the course is taught in Danish.

Remarks
The course replaces BMK21/BMKP21 in the old study programme, but it is no longer a PhD-course. The course will be taught in English if foreign students participate, otherwise in Danish. The number of participants is limited to 24.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.