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BMB526: Experimental molecular biology. Methods for investigation of eukaryotic gene expression. (10 ECTS)

STADS: 01004801

Level
Bachelor course

Teaching period
The course is offered in the autumn semester.
1st quarter.

Teacher responsible
Email: s.mandrup@bmb.sdu.dk

Timetable
Group Type Day Time Classroom Weeks Comment
Common I Monday 08-18 U26 og U27 31-33
Common I Tuesday 08-18 U26 og U27 31-33
Common I Wednesday 08-18 U56 og U64 31-33
Common I Thursday 08-18 U26 og U27 31-33
Common I Friday 08-18 U26 og U27 31-33
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Comment:
Undervisningen starter i uge 31. Max 38 studerende

Prerequisites:
None

Academic preconditions:
BMB503 Fundamental Biochemistry and BMB504 Fundamental Molecular Biology must be followed.

Course introduction
  1. To give the student theoretical knowledge of the molecular processes underlying differentiation of eukaryotic cells. In this case we focus on fat cell (adipocyte) differentiation.
  2. To give the student an experimental introduction to the commonly used methods for the analysis of the regulation of gene expression in mammalian cells.
  3. Through a project-oriented course to prepare to the students for their bachelor/ITEK/ speciale project.


Expected learning outcome
  1. Knowledge of differentiation of eukaryotic cells.
  2. Know-how of experimental procedures to analyse gene expression and the regulation threof in mammalian cells.
  3. Skills in research planning, interpretation and trouble shooting.
Subject overview
Theory:
Theoretical introduction to adipocyte differentiation with special focus on transcriptional regulation.
The role of the adipose tissue in insulin resistance, type 2 diabetes and the regulaiton of appetite.

Experimentally:
Purification of RNA
cDNA synthesis
Real-time qPCR
Extraction of nuclear proteins
Radioactive labelling of DNA oligos
Electrophoretic mobility shift assay
(Possibly more techniques suggested by the students)

Literature
There isn't any litterature for the course at the moment.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Project assignment based on lab. work. Pass/fail, internal evaluation by teacher.
Re-exam after 2nd quarter. The re-exam may have a different form than the ordinary exam.

Expected working hours
The teaching method is based on three phase model.

Forelæsninger (30 timer) og laboratorieøvelser (70 timer). Intensivt kursus med lange dage (10-12 timer) må forventes nogle af dagene.
Educational activities

Language
This course is taught in Danish.

Remarks
Course runs the first 2½ weeks of August. Intensive course with many long days. Calculate 2-3 weeks after the course to finish report. Deadline for handing in the report for comments is September 15, final deadline for handing in the report is November 1.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.