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Dansk English

BMB526: Experimental molecular biology. Methods for investigation of eukaryotic gene expression. (10 ECTS)

STADS: 01004801

Level
Bachelor course

Teaching period
The course is offered in the autumn semester.

Teacher responsible
Email: s.mandrup@bmb.sdu.dk

Timetable
Group Type Day Time Classroom Weeks Comment
Common I Monday 08-19 Green room 32
Common I Monday 08-19 Red Lab 32-33
Common I Monday 08-19 Red room 32-33
Common I Tuesday 08-19 Red room 32-33
Common I Tuesday 08-19 Green room 32
Common I Tuesday 08-19 Red Lab 32-33
Common I Wednesday 08-19 Red room 32-33
Common I Wednesday 08-19 Red Lab 32-33
Common I Thursday 08-19 Red Lab 32-33
Common I Thursday 08-19 Red room 32-33
Common I Friday 08-19 Red Lab 32-33
Common I Friday 08-19 Red room 32-33
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Comment:
Undervisningen starter onsdag den 29. juli. Sammenlæses med BMB826 - Max 38 studerende.

Prerequisites:
None

Academic preconditions:
BMB503 Fundamental Biochemistry and BMB504 Fundamental Molecular Biology or BMB532 Fundamental Biochemistry and BMB504 Fundamental Molecular Biology must be followed. The content of BMB508 Advanced Molecular Biologi must be known.

Course introduction
  1. To give the student theoretical knowledge of the molecular processes underlying differentiation of eukaryotic cells. In this case we focus on fat cell (adipocyte) differentiation.
  2. To give the student an experimental introduction to the commonly used methods for the analysis of the regulation of gene expression in mammalian cells.
  3. Through a project-oriented course to prepare to the students for their bachelor/ITEK/ speciale project.


Expected learning outcome
  1. Knowledge of differentiation of eukaryotic cells.
  2. Know-how of experimental procedures to analyse gene expression and the regulation threof in mammalian cells.
  3. Skills in research planning, interpretation and trouble shooting.
Subject overview
Theory:
Theoretical introduction to adipocyte differentiation with special focus on transcriptional regulation.
The role of the adipose tissue in insulin resistance, type 2 diabetes and the regulaiton of appetite.
introduction to deep-sequencing, bioinformatics and data generated by means of ChIP-seq

Experimentally:
Purification of RNA
cDNA synthesis
Real-time qPCR
Western blotting
ChIP
(Possibly more techniques suggested by the students)

Literature
    Artikler noter som lægges på e-learn


Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Project assignment based on lab. work. Pass/fail, internal evaluation by teacher.

Reexamination in the same exam period or immediately thereafter. The re-exam is held as an oral exam.

Expected working hours
The teaching method is based on three phase model.
Intro phase: 30 hours
Skills training phase: 70 hours, hereof:
 - Laboratory exercises: 70 hours

Educational activities

Language
This course is taught in Danish.

Remarks
Course runs the first 3 weeks of August. Intensive course with many long days. Calculate 2-3 weeks after the course to finish report. Deadline for handing in the report for comments is September 15, final deadline for handing in the report is November 1.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.