BMB807: THE SMALL RNA REGULATORS OF BACTERIA- Roles and Mechanisms in Gene Regulation (10 ECTS)

STADS: 01005001

Level
Master's level course

Teaching period
The course is offered in the autumn semester.
1st and 2nd quater

Teacher responsible
Email: valentin@bmb.sdu.dk

Timetable
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Comment:
Samlæses med BMB524.

Prerequisites:
None

Academic preconditions:
None

Course introduction


Expected learning outcome


Subject overview
• The expanding universe of non-coding RNAs . The history and range of ncRNAs in bacteria
• Identification of ncRNA:
The classic phase of ncRNA discovery
The modern phase of RNA hunting -Genomic mining
• Functional classes of ncRNAs:
RNAs that modify protein activity
RNAs that modulate translation
• Molecular mechanisms / Switching on and off with ncRNA
• Requirement for a conserved RNA binding protein, Hfq – a Sm like protein
• Structure and functions of Sm proteins –key players in RNA transactions.
• The physiological role of ncRNAs in Bacteria:
Control of sugar metabolism and storage of carbon
Regulation of cell adaptation: stress-induced sRNAs
Control of alternative sigma factors: The E and S paradigms.
Control in iron metabolism
Modulators of the bacterial surface
Role of Hfq and sRNAs in virulence
• Strategies for elucidating the function and/or targets for ncRNAs
Genearrays, proteomics and computer programs
• Antisense RNA control and its biotechnological application.
• Our current picture of antisense RNA action: Unsolved problems

Literature

• Noter: Riboregulation I bakterier. .

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
a) Lab. work, pass/fail, internal examination by the teacher. Active participation in laboratory exercises are a prerequisite to pass the course.
b) Oral examination, grades according to the Danish 7-scale, external examiner.

 

Expected working hours
The teaching method is based on three phase model.

Forelæsninger: 20 timer
Eksaminatorietimer/opgaveregning: 5 timer
Laboratorieøvelser: 70 timer
Educational activities

Language
No recorded information about the language used in the course.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.