BMB810: Human Recombinant Proteins in Molecular and Cell Biology (10 ECTS)

STADS: 01005801

Level
Master's level course

Teaching period
The course is offered in the spring semester.
3rd quarter.

Teacher responsible
Email: ogi@bmb.sdu.dk
Email: bag@bmb.sdu.dk

Timetable
Show entire timetable
Show personal time table for this course.

Comment:
Min. 5 deltagere og maks. 20 deltagere

Prerequisites:
None

Academic preconditions:
Bachelordegree in Biochemistry, Molecular Biology, Biomedicine and Biotechnology. The course cannot be followed by students that have passed BK55 or BMB523.

Course introduction
The course has been designed to acquaint the students with the most recent techniques in the fields og Molecular Biology and Cell Biology related to the design, expression and characterization of human recombinant protein kinases in bacteria and mammalian cells.

Expected learning outcome
Part a:
Design a strategy for the cloning of cDNA into bacteria expression plasmids.
Verify the successful cloning and expression of a recombinant protein kinase by SDS-PAGE and Western blot analysis.
Disign a strategy for the purification of the expressed protein kinase by means of chromatographic techniques.
Characterize the enzyme by its kinetic parameters by the application of radioactive and non-radioactive (luminometric) assays.
Part b:
Design a strategy for the cloning of CDNA into mammalian expression plasmids for the expression of a tag recombianat protein kinases.
Elucidate the intracellular regulation of the expression and activity of a protein kinase.
Determine the degree of expression by the application of biochemistry-based techniques (see part a) and fluorescence-based methods (fluorescence microscopy and fluorescence-activated cell sorting)

Subject overview
Cloning and expression of recombinant protein kinases in bacteria and mammalian cells and their purification and characterization by the application of biochemistry- and biophysic-based techniqes.

Literature
There isn't any litterature for the course at the moment.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
a) Written report based on lab. work. passed/failed, external censor.
b) Oral exam. Internal censor by the teachers. 7-point grading scale.

Re-exam after 4th quarter.

Expected working hours
The teaching method is based on three phase model.

Forlæsninger: 10
Laboratorieøvelser: 90

De studerende skal afholde små oplæg der forklarer teorien bag den eksperimentelle del.
Educational activities

Language
This course is taught in Danish or English, depending on the lecturer. However, if international students participate, the teaching language will always be English.

Remarks
The course is devided into two parts (i.e. Parts a and b) where the patecipation to Part a is mandatory for the participation to Part b.

Min. number of participants; 5;
max. number of participants: 20.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.