BMB811: Human Recombinant Proteins in Molecular and Cell Biology A (5 ECTS)

STADS: 01005901

Level
Master's level course

Teaching period
The course is offered in the spring semester.
3rd quarter

Teacher responsible
Email: ogi@bmb.sdu.dk
Email: bag@bmb.sdu.dk

Timetable
There is no timetable available for the chosen semester.

Prerequisites:
None

Academic preconditions:
Bachelor degree in Biochemistry, Molecular Biology, Biomedicine and Biotechnology. The course cannot be followed by students who have passed BK55 or BMB523.

Course introduction
The course has been designed to acquaint the students with the most recent techniques in the fields of Molecular Biology and Cell Biology related to the design, expression and characterization of human recombinant protein kinases in bacteria.

Expected learning outcome
Part a:
Design a strategy for the cloning of cDNA into bacteria expression plasmids.
Verify the successful cloning and expression of a recombinant protein kinase by SDS-PAGE and Western blot analysis.
Design a strategy for the purification of the expressed protein kinase by means of chromatographic techniques.
Characterize the enzyme by its kinetic parameters by the application of radioactive and non-radioactive (luminometric) assays.

Subject overview
Cloning and expression of recombinant protein kinases in bacteria and their purification and characterization by the application of biochemistry and biophysic-based techniques.

Literature
There isn't any litterature for the course at the moment.

Syllabus
See syllabus.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
a) Written report based on lab. work. External censor. 7-point grading scale.
The report has to be prepared by groups of two students.
b) Written exam. External censor. 7-point grading scale.

Re-exam after 4th quarter.
The form of the re-examination may differ from the ordinary evaluation.

Date of exam
The ordinary exam takes place on March 29, 2011
The re-examination takes place on June 27, 2011

Expected working hours
The teaching method is based on three phase model.

Lectures: 5
Laboratory exercises: 45

Students will be involved in oral presentations discussing the theory related to the experimental part.
Educational activities

Language
No recorded information about the language used in the course.

Remarks
The course is divided into two parts (i.e. part a and part b) where the participation in part b requires prior participation in part a.

Minimum number of participants: 5
Maximum number of participants: 20

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.