BMB812: Human Recombinant Proteins in Molecular and Cell Biology B (5 ECTS)

STADS: 01006001

Level
Master's level course

Teaching period
The course is offered in the spring semester.
3th quater

Teacher responsible
Email: ogi@bmb.sdu.dk
Email: bag@bmb.sdu.dk

Timetable
There is no timetable available for the chosen semester.

Prerequisites:
None

Academic preconditions:
Bachelor degree in Biochemistry, Molecular Biology, Biomedicine and Biotechnology, Participation in the course requires former participation in BMB811.

Course introduction
The course has been designed to acquaint the students wiht the most recent techniques in the fields of Molecular Biology and Cell Biology related to the design, expression and characterization of human recombinant protein kinases in mammalian cells.

Expected learning outcome
Design a strategy for the cloning of cDNA into mammalian expression plasmids for the expression of a tag recombinant protein kinase.
Elucidate the intracellular regulation of the expression and activity of a protein kinase. Determine the degree of expression by the application of biochemistry-based techniques (see part a) and fluorescence-based methods (fluorescence microscopy and fluorescence-activated cell sorting.)

Subject overview
Cloning and expression of recombinant protien kinases in mammalian cells, and characterization by the application of biochemistry- and biophysic-based techniques.

Literature
There isn't any litterature for the course at the moment.

Syllabus
See syllabus.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
a) Written report based on lab. work. External censor. 7-point grading scale.
b) Written exam. External censor. 7-point grading scale.

Re-exam after 4th quarter.
The form of the re-examination may differ from the ordinary evaluation.

Date of exam
The ordinary exam takes place on March 29, 2011
The re-examination takes place on June 27, 2011

Expected working hours
The teaching method is based on three phase model.

Lectures: 5
Laboratory exercises: 45

Students will be involved in oral presentations discussing the theory related to the experimental part.
Educational activities

Language
This course is taught in Danish or English, depending on the lecturer. However, if international students participate, the teaching language will always be English.

Remarks
The course is divided into two parts (i.e. part a and b) where the participation in part b requires prior participation in part a.

Minimum number of participants: 5
Maximum number of paticipants: 20

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.