FY518: Nanobioscience Methods (5 ECTS)

STADS: 07001901

Level
Bachelor course

Teaching period
The course is offered in the autumn semester.
2nd quarter.

Teacher responsible
Email: adam@memphys.sdu.dk

Timetable
Show entire timetable
Show personal time table for this course.

Prerequisites:
None

Academic preconditions:
Science year passed.

Course introduction
Introduction to important experimental techniques for characterization and preparation of biologicak nanostructures. The course consists of lectures, problems, demonstrations and hands-on experiments.

Expected learning outcome
At the end of the course the student must be able to:
1. Describe the principles of concrete nanobioscience techniques covered in the course.
2. Evaluate whether or not a given characterization technique is suitable for a specific purpose within nanobioscience.
3. Design an experiment and choose the relevant methods to carry it out.
4. Write a simple Labview program to interface with experimental hardware

Subject overview

• Basic and advanced use of optical microscopes and optical detection systems including: Microscope design, Objective types and digital CCD detection.
• Derivation of the resolution limit in classical optical microscopy.
• Contrast methods in the optical microscope including: Darkfield, Phase Contrast and Fluorescence
• Introduction to fluorescence microscopy in biology (epi, confocal and TIRF)
• Fluorescence spectroscopy and introduction to the fluorometer
• Encapsulation in liposomes. Release from liposomes
• High resolution scanning probe microscopy including Atomic Force Mciroscopy (AFM).
• Hand-on demonstration of the AFM
• Synthesis and characterization of fluorescent nanoparticles (Quantum Dots)
• Introduction to the programming language Labview for data acquisition, instrument control and data analysis. Programming exercises in Labview.
• Final project work based on individual research on an experimental technique for characterization or preparation of nanostructures.

Literature

• Noter, slides og opgaver som udleveres og er tilgængelige på Blackboard..

Syllabus
See syllabus.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Oral examination taking its atarting point in a written report (10-15 pages) which focuses on a specific technique or method of characterization. Furthermore the report must describe one or more esamples from the litterature on the uses of the described method. The student will give a 15 minute oral presentation based on the report followed by questioning.
Graded with pass/fail and internal marking.

Re-examination after 4th quarter.

Date of exam
The ordinary exam takes place on January 4, 2011
The re-examination takes place on June 29, 2011

Expected working hours
The teaching method is based on three phase model.

Lectures: 15 hours.
Tutorials: 15 hours.
Laboratory exercises: 10 hours.
Excursion: 10 hours.
Educational activities

Language
This course is taught in Danish.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.