FY518: Nanobioscience Methods (5 ECTS)

STADS: 07001901

Level
Bachelor course

Teaching period
The course is offered in the autumn semester.
2nd quarter.

Teacher responsible
Email: adam@memphys.sdu.dk

Timetable
Show entire timetable
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Comment:
Obligatorisk kursus på Nanobioscience studieretningen. Kører i forlængelse af FY514 Nanobioscience-systemer. Afvikles med 2x2 timers klasseundervisning per uge samt demonstrationsøvelser og afsluttende individuel projektskrivning. Kan tages som valgfrit kursus af andre interesserede fysik- eller kemistuderende. Kontakt Adam Cohen Simonsen for yderligere spørgsmål.

Prerequisites:
None

Academic preconditions:
Science year passed.

Course introduction
Introduction to important experimental techniques for characterization and preparation of biologicak nanostructures. The course consists of lectures, problems, demonstrations and hands-on experiments.

Expected learning outcome
At the end of the course the student must be able to:
1. Describe the principles of concrete nanobioscience techniques covered in the course.
2. Evaluate whether or not a given characterization technique is suitable for a specific purpose within nanobioscience.
3. Design an experiment and choose the relevant methods to carry it out.
4. Write a simple Labview program to interface with experimental hardware

Subject overview

• Basic and advanced use of optical microscopes and optical detection systems including: Microscope design, Objective types and digital CCD detection.
• Derivation of the resolution limit in classical optical microscopy.
• Contrast methods in the optical microscope including: Darkfield, Phase Contrast and Fluorescence
• Introduction to fluorescence microscopy in biology (epi, confocal and TIRF)
• Fluorescence spectroscopy and introduction to the fluorometer
• Encapsulation in liposomes. Release from liposomes
• High resolution scanning probe microscopy including Atomic Force Mciroscopy (AFM).
• Hand-on demonstration of the AFM
• Synthesis and characterization of fluorescent nanoparticles (Quantum Dots)
• Introduction to the programming language Labview for data acquisition, instrument control and data analysis. Programming exercises in Labview.
• Final project work based on individual research on an experimental technique for characterization or preparation of nanostructures.

Literature

• Noter, slides og opgaver som udleveres og er tilgængelige på Blackboard..

Syllabus
See syllabus.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Oral examination taking its atarting point in a written report (10-15 pages) which focuses on a specific technique or method of characterization. Furthermore the report must describe one or more esamples from the litterature on the uses of the described method. The student will give a 15 minute oral presentation based on the report followed by questioning.
Graded with pass/fail and internal marking.

Re-examination after 4th quarter.

Expected working hours
The teaching method is based on three phase model.

Forelæsninger, antal timer 15.
Eksaminatorietimer/opgaveregning, antal timer 15.
Laboratorieøvelser, antal timer 10.
Ekskursion, antal timer 10.
Educational activities

Language
This course is taught in Danish.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.