BMB526: Experimental molecular biology. Methods for investigation of eukaryotic gene expression. (10 ECTS)

STADS: 01003401

Level
Bachelor course

Teaching period
The course is offered in the spring semester.
4th quarter.

Teacher responsible
Email: s.mandrup@bmb.sdu.dk

Timetable
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Comment:
Max: 38 studerende

Intensivt kursus i de første 2½ uge af august.
Tilmeldes som forårskursus.

Prerequisites:
None

Academic preconditions:
BMB503 Fundamental Biochemistry and BMB504 Fundamental Molecular Biology must be followed.

Course introduction
1. To give the student theoretical knowledge of the molecular processes underlying differentiation of eukaryotic cells. In this case we focus on fat cell (adipocyte) differentiation.
2. To give the student an experimental introduction to the commonly used methods for the analysis of the regulation of gene expression in mammalian cells.
3. Through a project-oriented course to prepare to the students for their bachelor/ITEK/ speciale project.

Expected learning outcome
1. Knowledge of differentiation of eukaryotic cells.
2. Know-how of experimental procedures to analyse gene expression and the regulation threof in mammalian cells.
3. Skills in research planning, interpretation and trouble shooting.

Subject overview
Theory:
Theoretical introduction to adipocyte differentiation with special focus on transcriptional regulation.
The role of the adipose tissue in insulin resistance, type 2 diabetes and the regulaiton of appetite.

Experimentally:
Purification of RNA
cDNA synthesis
Real-time qPCR
Extraction of nuclear proteins
Radioactive labelling of DNA oligos
Electrophoretic mobility shift assay
(Possibly more techniques suggested by the students)

Literature

• Øvelsesvejledning.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
a) Project assignment. Pass/fail, internal evaluation by teacher.
b) Lab. work. Pass/fail, internal evaluation by teacher.

Expected working hours
The teaching method is based on three phase model.

Forelæsninger (30 timer) og laboratorieøvelser (70 timer). Intensivt kursus med lange dage (10-12 timer) må forventes nogle af dagene.
Educational activities

Language
This course is taught in Danish.

Remarks
Course runs the first 2½ weeks of August. Intensive course with many long days. Deadline for handing in report is October 1. Calculate 1-2 weeks after the course to finish report.

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.