BMB529: (5 ECTS)

STADS: 01007301

Level
Bachelor course

Teaching period
The course is offered in the spring semester.

Teacher responsible
Email: php@bmb.sdu.dk

Additional teachers
hjernoe@bmb.sdu.dk
lipen@bmb.sdu.dk

Timetable
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Comment:
Max deltagere 12

Prerequisites:
None

Academic preconditions:
Knowledge of the content of BMB506 Protein Chemistry

Course introduction
The purpose of the course isto give theh student a basic introduction in protein chemical techniques which are used to charaterization of the primary structure of proteins and their identification

Expected learning outcome
Statement of Aims:

Describe the primary structure of a protein and explain the principles in the most importan methods for primary structure analysis
• Explain the principles in separation of polypeptides by HPLC
• Describe the principles in gel electrophoresis
• Explain the principles in amino acid analysis, Edman degradation and mass spectrometry of polypeptides, be able to analyze the related data and integrate these to solve the primary structure of a protein
• Explain the principle of a MALDI mass spectrometer
• Explain the principles in mass spectrometric protein identification and be able to analyze the related data.
• Explain the principles in mass spectrometric sequencing of peptides and analyze ms/ms data.
• To evaluate the techniques used in the course for analysis of protein structure.
• To use the computer programs available for the course  

Subject overview
The following topics will be treated during the course:
1. Primary structure of proteins
2. Chemical characteristics of amino acids
3. The principles of amino acid analysis and its use for
 a) Protein / peptide characterization
 b) Quantitation
4. Protein sequencing in the form of:
 a) Edman degradation
 b) Mass spectrometric sequencing
5. SDS gel electrophoresis of proteins
6. Liquid chromatography
 a) Reverses phase for peptide separation
 b) Ion exchange
 c) Solid phase extraction
7. Basic mass spectrometry (MALDI)
 a) Intact mass analysis
 b) Peptide mass fingerprinting
 c) MS/MS for identification
8. Bioinformatical proteinanalysis     

Literature

• Øvelsesvejledning.

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Project assignment (5 ECTS), which is to be handed in three weeks after end of course. Pass/fail, internal examiner
 

Expected working hours
The teaching method is based on three phase model.

Undervisningen vil bestå af forelæsninger (10 timer), eksaminatorietimer/fremlæggelse (6 timer) og laboratorieøvelser/databehandling (40 timer)

(De angivne timetal er vejledende, der vil kunne forekomme afvigelser)
Educational activities

Language
This course is taught in Danish.

Remarks
If failed, the report can be resubmitted after two weeks

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.