BMB534: (5 ECTS)

STADS: 01011401

Level
Bachelor course

Teaching period
The course is offered in the spring semester.
4th quarter

Teacher responsible
Email: php@bmb.sdu.dk

Timetable
Show entire timetable
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Prerequisites:
None

Academic preconditions:
BMB506 Protein Chemistry - Passed

 

Course introduction
The purpose of the course is to give the student a introduction in basic protein chemical techniques which are used for charaterization of the primary structure of proteins and their identification.

Expected learning outcome
Statement of Aims:

• Describe the primary structure of a protein and explain the principles in the most importan methods for primary structure analysis
• Explain the principles in separation of polypeptides by HPLC
• Describe the principles in gel electrophoresis
• Explain the principles in amino acid analysis, Edman degradation and mass spectrometry of polypeptides, be able to analyze the related data and integrate these to solve the primary structure of a protein
• Explain the principle of a MALDI mass spectrometer
• Explain the principles in mass spectrometric protein identification and be able to analyze the related data.
• Explain the principles in mass spectrometric sequencing of peptides and analyze ms/ms data.
• To evaluate the techniques used in the course for analysis of protein structure.
• To use the computer programs available for the course  



Subject overview
The following topics will be treated during the course:
1. Primary structure of proteins
2. Chemical characteristics of amino acids
3. The principles of amino acid analysis and its use for
 a) Protein / peptide characterization
 b) Quantitation
4. Protein sequencing in the form of:
 a) Edman degradation
 b) Mass spectrometric sequencing
5. SDS gel electrophoresis of proteins
6. Liquid chromatography
 a) Reverses phase for peptide separation
 b) Ion exchange
 c) Solid phase extraction
7. Basic mass spectrometry (MALDI)
 a) Intact mass analysis
 b) Peptide mass fingerprinting
 c) MS/MS for identification
8. Bioinformatical protein analysis 

Literature

Oplyses senere

Website
This course uses e-learn (blackboard).

Prerequisites for participating in the exam
None

Assessment and marking:
Project assignment. Pass/fail, internal examiner. Course report to be handed in three weeks after end of course. (01011402)

If failed, the report can be resubmitted after two weeks   

Expected working hours
The teaching method is based on three phase model.

Forelæsning, 8 timer
Eksaminatorier, 4 timer
Laboratorieøvelser, 35 timer

Det præcise forhold mellem undervisningsformer kan variere

 
Educational activities

Language
This course is taught in Danish or English, depending on the lecturer. However, if international students participate, the teaching language will always be English.

Remarks
If failed, the report can be resubmitted after two weeks

Course enrollment
See deadline of enrolment.

Tuition fees for single courses
See fees for single courses.